RNA

Part:BBa_K3289024:Design

Designed by: Tatiana Houhou   Group: iGEM19_NYU_Abu_Dhabi   (2019-10-21)


crRNA HbcAg


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 34
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For designing the gRNAs, we used benchling. For each DNA sequence we first created our primers (PCR AND RPA) and attached them to our DNA strands in order to be able to visualize them on the software's linear map. Making sure that the RPA and LAMP primers binding sequences are nested within the PCR amplicon, we then selected as our target an appropriate region that falls within our RPA amplicon in order to create our guide RNAs. When multiple crRNA options were provided, the ones with the highest off-target score were selected. The selected crRNA were then NCBI blasted to make sure that they are specific to the chosen bacterial strain.


Source

This part was synthesized directly from IDT.

References